Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2016/07/31

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U2OS Transient transfection (KAH132) Microscopy Results

U2OS ΔTF (KAH132) transfection results.

Post 24 hours

Well 1:
2016-07-29 Well 1 KAH132-MV2 phase mCherry 10x.jpg


Well 2:
2016-07-29 Well 2 KAH132-MV2 phase mCherry 10x.jpg


Well 3:
2016-07-29 Well 3 KAH132-MV2 phase mCherry 10x.jpg


Well 4:
2016-07-29 Well 4 KAH132-MV2 phase mCherry 10x.jpg


Well 5:
2016-07-29 Well 5 KAH132-MV2 phase mCherry 10x.jpg


Well 6:
2016-07-29 Well 6 KAH132-MV2 phase mCherry 10x.jpg



Post 48 hours

Well 2:
2016-07-30 Well 2 KAH132-MV2 phase mCherry 10x.jpg


Well 3:
2016-07-30 Well 3 KAH132-MV2 phase mCherry 10x.jpg


Well 5:
2016-07-30 Well 5 KAH132-MV2 phase mCherry 10x.jpg


Well 6:
2016-07-30 Well 6 KAH132-MV2 phase mCherry 10x.jpg



Post 72 hours

Well 3:
2016-07-31 Well 3 KAH132-MV2 phase mCherry 10x.jpg


Well 6:
2016-07-31 Well 6 KAH132-MV2 phase mCherry 10x.jpg


U2OS Transient transfection (KAH132) Flow Cytometry Results

2016-07-29 U2OS KAH132 transfection 24hr flow data.png


2016-07-30 U2OS KAH132 transfection 48hr flow data.png


2016-07-31 U2OS KAH132 transfection 72hr flow data.png

Transfection efficiencies:

Sample ID % RFP
24hr A 45.3
24hr B 48.3
48hr A 43.7
48hr B 48
72hr A 31.7
72hr B 29.7