Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/09/01
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I'm going to be performing a series of transfections with the following parameters:
Vector used: KAH126-MV2
U-2 OS Transfection
Earlier in the week, I set up a 6-well plate with U-2 OS cells, and today I transfected the plate with KAH126-MV2 using Lipofectamine LTX. Wells 1, 2, 4, and 5 were treated with 4 µg of plasmid, and wells 3 and 6 were negative controls (no plasmid). After transfection, the plate was centrifuged at 128 g for 30 minutes at room temperature before being transferred to the CO2 incubator.