Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/06/16
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Checked concentration of RNA from RNeasy preps performed last week. Expected 260/280 ratio for pure RNA is ~2.0.
DNASU needs 50-100 ng of RNA in 5 µL total volume (10-20 ng/µL conc.). So I'll need to make some dilutions first before submitting anything to them.