Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/06/13

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Overview

Collecting the second timepoint for the no plasmid control.

Microscopy

Well 4: no plasmid control, post-48 hrs
2015-06-13 Well 4 no plasmid phase 10x.jpg

Flow Cytometry

Plot 1 (FSC-A/SSC-A) Plot 4 (FL3-A) Plot 6 (FL1-A)
All Cells M1 M2
Count Volume (µL) Events / µL Count Events / µL % of All Count % of This Plot Count % of This Plot
A02 4 no plasmid 43,616 10 4362 31,378 3138 71.94% 133 0.42% 57 0.18%

RNA Miniprep

Harvested well 4 and treated with Trizol, storing at -80 °C until more Qiagen RNeasy kits arrive.