Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/06/12

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Overview

Collecting the first timepoint for the no plasmid control. Will collect the post-48hr sample tomorrow.

Microscopy

Well 1: no plasmid control, post-24 hrs
2015-06-12 Well 1 no plasmid phase 10x.jpg

Well 4: no plasmid control, post-48 hrs
2015-06-12 Well 4 no plasmid phase 10x.jpg

Flow Cytometry

Plot 1 (FSC-A/SSC-A) Plot 4 (FL3-A) Plot 6 (FL1-A)
All Cells M1 M2
Count Volume (µL) Events / µL Count Events / µL % of All Count % of This Plot Count % of This Plot
A01 Blank 141 10 14 0 0 0.00% 0 100.00% 0 100.00%
A02 1 no plasmid 15,552 10 1555 11,704 1170 75.26% 37 0.32% 17 0.15%
A03 4 no plasmid 17,218 10 1722 13,026 1303 75.65% 30 0.23% 15 0.12%

RNA Miniprep

Harvested well 1 and treated with Trizol, storing at -80 °C until more Qiagen RNeasy kits arrive.