Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/06/11

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Overview

Culture density of K562: 870,000 cells/mL. To do two mock transfections (plus buffer), need a total of 5,000,000 cells, or 5.75 mL of culture, spun down, resuspended in 5 mL PBS, spun down and resuspended again in 250 µL Resuspension Buffer R. Also passage cells.