Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/06/05

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Overview

Cell density measured at 1,100,000 cells/mL via hemacytometer.

Will dilute to 150,000 cells/mL today for growth over the weekend.

1.5/11 * 20 = 2.7 mL culture 20-2.7 = 17.3 mL fresh medium