Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/06/02

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.pngDNA concentration of KAH126-MV2 Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Overview

I currently have a large stock of KAH126-MV2 at 809 ng/µL. I want to concentrate it further to about 2400 ng/µL. Will follow the procedure given in the GenElute HP Endotoxin-Free Plasmid Maxiprep Kit.

Starting volume of plasmid is 300 µL, final volume is 100 µL.

DNA Concentration

Sample ID Conc. (ng/µL) st dev (ng/µL) A260/A280
KAH126-MV2 original 642.4 26.43 1.91
KAH126-MV2 precip 2256.7 35.76 1.90
KAH126-MV2 1:10 dil 166.5 4.18 1.78

Final concentration is between 1660 ng/µL and 2257 ng/µL. I'm going to guess it's about 2000 ng/µL.