Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/27
|Neon transfection of K562 cell line||Main project page|
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Trying transfection of K562 again, this time using the Neon transfection system.
Treat samples in duplicate, for 12 samples in total.
Culture density: 240,000 cells/mL * 5 mL culture = 1.2E6 cells total
To make 1E7 cells/mL concentration, spin down and resuspend in 120 µL Resuspension Buffer R.
pMaxGFP concentration: 2000 ng/µL
KAH126-MV2 concentration: 809 ng/µL