Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/26
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There appears to be a direct correlation between amount of DNA used and transfection efficiency. All recorded efficiencies were very low, however (<5%). Amount of LTX does not appear to have a significant effect, although this is difficult to verify as no samples used a constant amount of DNA while varying lipofectamine.