Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/26

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Owwnotebook icon.pngK562 transfection optimization results Report.pngMain project page
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Overview

Sample ID DNA (µg) LTX (µL) % transfection (avg) % transfection (st dev)
1 4 2 2.81% 0.004949747
2 1 2 1.50% 0.002404163
3 0.4 2 0.50% 0.000636396
4 6 3 1.90% 0.003676955
5 1.5 3 1.86% 0.000494975
6 0.6 3 0.77% 0.000282843
7 8 4 4.15% 0.003676955
8 2 4 1.52% 0.003889087
9 0.8 4 1.12% 0.000565685
10 10 5 4.47% 0.001626346
11 2.5 5 2.53% 0.002192031
12 1 5 2.53% 0.013010765
0 0 0 0.08% 0.000494975

2015-05-26 K562 test transfection image 1.png

2015-05-26 K562 test transfection image 2.png

There appears to be a direct correlation between amount of DNA used and transfection efficiency. All recorded efficiencies were very low, however (<5%). Amount of LTX does not appear to have a significant effect, although this is difficult to verify as no samples used a constant amount of DNA while varying lipofectamine.