Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/22

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Overview

Measured cell density of K562 culture in antibiotic-free medium at 490,000 cells/mL. At 9 mL total culture, that's 4,410,000 cells total. Ideal cell density for transfection with lipofectamine in 24-well plate is 0.5-2E5 cells per well. Assume 1 extra mL of culture as buffer.

4.90E5 cells/mL * x mL = 0.5E5 cells/mL * 25 mL

x = 12.5E5 cells / (4.9E5 cells/mL)

x = 2.55 mL

25 - 2.55 = 22.45 mL medium