Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/18

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Overview

Here's the images taken 3 days after transfection of K562 cells.

KAH187-MV8 (positive control):
2015-05-18 Well 1 KAH187-MV8 CFP phase 10x.jpg 2015-05-18 Well 4 KAH187-MV8 CFP phase 10x.jpg

KAH126-MV2:
2015-05-18 Well 2 KAH126-MV2 mCherry phase 10x.jpg 2015-05-18 Well 5 KAH126-MV2 mCherry phase 10x.jpg

KAH87-MV10:
2015-05-18 Well 3 KAH87-MV10 mCherry phase 10x.jpg 2015-05-18 Well 6 KAH87-MV10 mCherry phase 10x.jpg

Almost no transfection of the test plasmids, and low transfection of the positive control. Could be due to high passage number (was on PS-9). Or cell density is less than optimal. Need to design an optimization experiment.