Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/15
|Transfection of K562 using KAH187-MV8, KAH126-MV2, KAH87-MV10||Main project page|
Previous entry Next entry
Repeating last week's experiment with K562 and a working copy of the KAH87-MV10 plasmid.
See the Haynes Lab transfection protocol for details.
One difference: final volume of antibiotic-free medium in each well is 2mL, not 4mL. All other values kept the same.
Cell density as measured by hemacytometer is 460,000 cells per well.