Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/11

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Overview

U-2 OS culture was transfected on Friday, May 8. I'm now checking on the success of those transfections.

There were three transfections performed (in duplicate): KAH187-MV8 (positive control, CFP); KAH126-MV2 (RFP); and KAH87-MV10 (RFP).

Images

KAH187-MV8

2015-05-11 Well 1 KAH187-MV8 phase CFP 10x.jpg 2015-05-11 Well 4 KAH187-MV8 phase CFP 10x.jpg


KAH126-MV2

2015-05-11 Well 2 KAH126-MV2 phase mCherry10x.jpg 2015-05-11 Well 5 KAH126-MV2 phase mCherry10x.jpg

KAH87-MV10

2015-05-11 Well 3 KAH87-MV10 phase mCherry10x.jpg 2015-05-11 Well 6 KAH87-MV10 phase mCherry10x.jpg

Results

Positive control worked as expected. Surprisingly, KAH126 did as well; might be due to low cell density prior to transfection (~70% confluency). KAH87-MV10 did not show any transfection efficiency - this is most likely due to the fact that the insert in the plasmid was placed backwards. Will repeat KAH87-MV10 once I have a better copy.