Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/08
|Test transfection of U2OS using KAH187-MV8, KAH126-MV2, and KAH87-MV10||Main project page|
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Repeating the transfection from earlier, this time trying out a new plasmid made by Dr. Haynes: KAH87-MV10.
Each treatment will use be performed in duplicate on a 6-well plate.
See the Haynes Lab transfection protocol for details.
One difference: final volume of antibiotic-free medium in each well is 2mL, not 4mL. All other values kept the same.