Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/05
|Test transfection of U2OS using pMaxGFP, KAH187-MV8, and KAH126-MV2||Main project page|
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Transfection efficiency has been much lower than anticipated despite numerous attempts to optimize. Here I'm going to try transfection with three different plasmids: pMaxGFP (positive control from Lonza), KAH187-MV8 (expresses CFP using CMV promoter), and KAH126-MV2 (expresses PcTF-RFP using CMV promoter). Will see how they differ in effectiveness.
Each treatment will use be performed in duplicate on a 6-well plate.
See the Haynes Lab transfection protocol for details.
One difference: final volume of antibiotic-free medium in each well is 2mL, not 4mL. All other values kept the same.