Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/04/15
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Plasmid Maxiprep of KAH187-MV8
--Notes-- During the plasmid purification procedure, I accidentally mixed the lysate flowthrough (containing plasmid) with the column preparation solution that was run through the Binding Column. Proceeding as normal from this point forward. Hopefully doesn't influence yield.
Measured using the Take3 plate:
--Restriction Enzyme Digest--
Digest for 15 minutes at 37°C.
Expected size fragments from EcoRI/ApaLI digestion: 3951, 1243, 1135, 288, 234.
Gel was run a little too long, ~300bp fragment sizes are difficult to visualize.