Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/03/11

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In paragraph format, state what you are going to do today so a reader unfamiliar with your project could understand what is going on.


  • ✓ measure K562 concentration using flow cytometry
  • ✓ transfect K562 with KAH126-MV2 plasmid using lipofectamine 3000
    • ✓ take care to use correct cell density, and do not disturb the cells after adding lipofectamine mixture.


example protocol


Observations, measurements, placeholder info etc. go here.


K562 culture concentration: 516,000 cells/mL

To get to 500,000 cells total per well, dilute 4-fold in fresh medium. 6mL culture, 18mL medium.