Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/03/10

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Summary

In paragraph format, state what you are going to do today so a reader unfamiliar with your project could understand what is going on.



Goals

  • ✓ measure K562 concentration using flow cytometry



Protocol

example protocol




Notes

Observations, measurements, placeholder info etc. go here.



Results/Conclusions

Cell density of K562 culture is 212,000 cells/mL.

Looking at the lipofectamine 3000 protocol, it lists the ideal number of adherent cells (for 6-well plate) at 0.25 - 1 x 10^6, total. Therefore, since there's 4mL of culture per well, the ideal cell concentration should be 6.25 - 25 x 10^6 cells/mL. Will have to dilute the culture before transferring to 6-well plate.