Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/02/18

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Owwnotebook icon.pngRNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines Report.pngMain project page
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In paragraph format, state what you are going to do today so a reader unfamiliar with your project could understand what is going on.


  • ✓ Take photographs of transfected K562 cells


example protocol


Observations, measurements, placeholder info etc. go here.


Fluorescence photos:

Well 1 (positive control, GFP)

Well 2 (PcTF-RFP, replicate 1)

Well 3 (PcTF-RFP, replicate 2)

Positive control worked great, but samples did not appear to have significant levels of transfection. Will have to troubleshoot from here - see if there's a way to improve plasmid quality?