Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/02/10

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Summary

I'm submitting four RNA samples to DNASU today. They are:

SKNSH: transfected with KAH126/MV2 SKNSH: negative control U2OS: transfected with KAH126/MV2 U2OS: negative control

The SKNSH cells were transfected on 2/5 and harvested on 2/9. The U2OS cells were transfected on 1/22 and harvested on 1/27.



Goals

  • ✓ Quantify RNA in samples
  • ✓ Submit samples to DNASU



Protocol

example protocol




Notes

Observations, measurements, placeholder info etc. go here.



Results/Conclusions

Plate reader data for RNA samples:

SKNSH, KAH126/MV2: 548.4515 ng/μL
SKNSH, neg control: 440.6735 ng/μL
U2OS, KAH126/MV2: 662.9805 ng/μL
U2OS, neg control: 728.3075 ng/μL

Full data:

Sample ID Replicate 1 Replicate 2 Average Reading
SKNSH transfected w/ KAH126/MV2 0.69 0.681 0.6855 260
0.331 0.327 0.329 280
2.081 2.086 2.0835 260/280
551.82 545.083 548.4515 ng/µL
SKNSH negative control 0.562 0.54 0.551 260
0.27 0.26 0.265 280
2.078 2.076 2.077 260/280
449.5 431.847 440.6735 ng/µL
U2OS transfected w/ KAH126/MV2 0.862 0.795 0.8285 260
0.415 0.385 0.4 280
2.078 2.066 2.072 260/280
689.651 636.31 662.9805 ng/µL
U2OS negative control 0.895 0.926 0.9105 260
0.426 0.443 0.4345 280
2.102 2.091 2.0965 260/280
715.765 740.85 728.3075 ng/µL