Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/02/09
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Following up on the transfection of SKNSH cells last Thursday, today I'm going to image them again and make a decision on whether to harvest for RNA-seq. Also need to start a culture of K-562 cells in preparation for transfecting them with PcTF-RFP.
Observations, measurements, placeholder info etc. go here.
Fluorescence microscopy images:
Harvested the cells (2 biological replicates, 1 control), RNA is in the -80°C freezer.
K562 culture is in PS-α, in the incubator.
Potential plasmids to use as positive control in transfection/nucleofection: KAH133 (mCherry in MV2), KAH187 (CFP in MV8)