Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/02/05

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Owwnotebook icon.pngRNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines Report.pngMain project page
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Summary

SKNSH cells in 6-well plate are ready for transfection, so today I'll be doing that (again). Also preparing more U2OS medium with 10% FBS and 1% pen/strep, and passaging KAH126/U2OS and SKNSH cultures.



Goals

  • Make more U2OS medium (McCoy's 5A w/ 10% FBS, 1% P/S)
  • Transfect SKNSH cells in 6-well plates
  • Passage KAH126/U2OS
  • Passage SKNSH



Protocol




Notes

Observations, measurements, placeholder info etc. go here.



Results/Conclusions

Data collected from your experiments, significance of findings, future steps etc. all go here. Like the introduction of the page, this should also be in paragraph format and summarize your findings so a new reader can follow along.