Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/01/22
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Today the U2OS culture in 6-well plates looks ready for transfection (~80% confluency) and the KAH126/U2OS culture in 6-well plates looks ready for induction by doxycycline (~80% confluency). I'll be taking care of that, as well as running a plasmid mini-prep on some E. coli cells I grew up and harvested earlier in the week.
Observations, measurements, placeholder info etc. go here.
Fluorescence microscopy images of KAH126/U2OS culture (taken today, induced on Monday) are below.
DNA concentration data from plasmid mini-prep:
Average concentration of plasmid from colony 1: 96.163 ng/μL
Average concentration of plasmid from colony 2: 59.2485 ng/μL
NOTE: All of the plasmid collected from colony 1 was consumed in the transfection of the U2OS cells today. The wells transfected with the new plasmid were #'s 3 and 4.