Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/01/20

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Owwnotebook icon.pngRNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines Report.pngMain project page
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Summary

In order to maintain cell cultures and prepare some plates for transfection, I'm going to passage the U2OS and KAH126/U2OS cultures and replace the medium in the SKNSH culture.



Goals

  • Replace medium in SKNSH culture
  • Passage/split U2OS
  • Passage/split KAH126/U2OS



Protocol

splitting/passaging cells




Notes

Observations, measurements, placeholder info etc. go here. Like the introduction of the page, this should also be in paragraph format and summarize your findings so a new reader can follow along.



Results/Conclusions

New plates and flasks are in the incubator in the tissue culture room.