Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/01/16
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U2OS, KAH126/U2OS and SKNSH cultures are ready to be expanded/passaged. Also going to try making more KAH126/MV2 plasmid.
Observations, measurements, placeholder info etc. go here
SKNSH was diluted 2-fold when expanded into the T-75 from the T-25. This should reduce the time period between passages and hopefully eliminate the differentiation of cells into new morphology.
KAH126/U2OS cells were split into a 6-well plate. They were diluted 10-fold before splitting.
U2OS cells were expanded, and are in the incubator.
Bacterial cells are taking a while to grow, will need to check on them tomorrow morning.