Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/01/15

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Owwnotebook icon.pngRNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines Report.pngMain project page
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Summary

Not much going on today, besides checking on cultures and preparing materials for the incoming student volunteers.



Goals

  • Check U2OS, SKNSH, and KAH126/U2OS cultures for confluency



Protocol

example protocol




Notes

Observations, measurements, placeholder info etc. go here



Results/Conclusions

SKNSH and KAH126/U2OS cultures are both at around 10-20% confluency. They'll need another couple of days before they can be split or expanded.

The U2OS culture is at over 60% confluency. It's ready to be expanded from PS-α to PS-β tomorrow.