Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/01/07

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Owwnotebook icon.pngRNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines Report.pngMain project page
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Summary

Quick entry here because I'm heading home and need to get out the door -

  • Transfected SKNSH cells with KAH126/MV2 plasmid using lipofectamine method
  • Picked 2 DH5α colonies from the LB-Amp plate and streaked them on a new plate, then placed the streaking tip of each colony into a 5mL round-bottom tube with LB-Amp in the 37C shaker incubator (Wang lab). Will check on culture status tomorrow AM.



Goals

  • Place main goals for the day here



Protocol

example protocol



Materials

  • List materials here, to be gathered before the experiment
  • Consumables such as media, antibiotics, DNA, kits etc.



Notes

Observations, measurements, placeholder info etc. go here



Results/Conclusions

Any significant data goes here. Thoughts on what happened, significance of findings, what to do next etc.