Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/01/07

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Owwnotebook icon.pngRNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines Report.pngMain project page
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Quick entry here because I'm heading home and need to get out the door -

  • Transfected SKNSH cells with KAH126/MV2 plasmid using lipofectamine method
  • Picked 2 DH5α colonies from the LB-Amp plate and streaked them on a new plate, then placed the streaking tip of each colony into a 5mL round-bottom tube with LB-Amp in the 37C shaker incubator (Wang lab). Will check on culture status tomorrow AM.


  • Place main goals for the day here


example protocol


  • List materials here, to be gathered before the experiment
  • Consumables such as media, antibiotics, DNA, kits etc.


Observations, measurements, placeholder info etc. go here


Any significant data goes here. Thoughts on what happened, significance of findings, what to do next etc.