Happy New Year! Let's do some science.
The U2OS culture is at optimal density, so I'll be performing another transfection today. Also continuing to measure growth on the 24-well plate using flow cytometry, and advancing the KAH126/U2OS culture from PS-α to PS-β.
Goals
Transfect 3 U2OS cultures from 6-well plate with PcTF-RFP plasmid (and use 1 well as negative control)
Take photographs of cultures to show confluency levels
Measure cell density on 3 U2OS cultures from 24-well plate using flow cytometry
Observations, measurements, placeholder info etc. go here
Results/Conclusions
Photograph of the culture is on the right. Near-total confluency observed.
Below is the table data from flow cytometry of the 24-well samples.
All
P2
Count
Volume (µL)
Events / µL
Count
Events / µL
% of All
A01 Blank
635
100
6
0
0
0.00%
A02 Well A6
7,381
100
74
7,381
74
100.00%
A03 Well C1
7,734
100
77
7,734
77
100.00%
A04 Well D2
6,814
100
68
6,814
68
100.00%
Average cell density of 73,000 +/- 4600 cells/mL is observed. This translates to a cell count of approximately 292,000 cells per well in the 6-well plate when transfection occurred.
KAH126/U2OS cell line was not advanced due to lack of T-75 culture flasks. Will try again tomorrow and see about borrowing flasks from another lab.