Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2014/12/31
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Repeat of yesterday's work.
Checking on the U2OS & SKNSH cell lines today. I'll take three wells from the 24-well plate and 1 well from the 6-well plate, trypsinize the cells to get them into solution, then measure their density on the flow cytometer. Should be able to get a rough idea of when the plates will be ready for transfection then.
I'll also be taking pictures of the plates in order to correlate measured cell density (via FC) with estimated confluence.
Observations, measurements, placeholder info etc. go here
Flow cytometry data:
U2OS culture confluency on the right:
It appears cell density is somewhere between 150,000 cells (in 4mL of medium) and 100,000 cells (in 1 mL of medium, corrected for volume). Will perform transfection tomorrow.