Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2014/12/22
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Here are some pictures taken of the cells after the second transfection attempt. Efficiency is still low, but we'll be expanding the lines regardless.
SKNSH cells to be passaged from PS-β to PS-1, 5-fold dilution.
Composite photographs (phase contrast & mCherry red fluorescence) of U2OS cultures transfected with PcTF-mCherry plasmid: