Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2014/12/22

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Owwnotebook icon.pngRNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines Report.pngMain project page
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Summary

Here are some pictures taken of the cells after the second transfection attempt. Efficiency is still low, but we'll be expanding the lines regardless.

Goals

  • Expand U2OS transfected cell lines (from 2nd attempt)
  • Check SKNSH cell line, passage if necessary

Protocol

passaging cells

Materials

  • List materials here, to be gathered before the experiment
  • Consumables such as media, antibiotics, DNA, kits etc.

Notes

SKNSH cells to be passaged from PS-β to PS-1, 5-fold dilution.

Results/Conclusions

Composite photographs (phase contrast & mCherry red fluorescence) of U2OS cultures transfected with PcTF-mCherry plasmid:

20141222 Well 1 10x phase & mCherry.jpg

20141222 Well 2 10x phase & mCherry.jpg

20141222 Well 3 10x phase & mCherry.jpg

20141222 Well 4 neg control 10x phase & mCherry.jpg