Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2014/12/11

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Starting cell lines

Goals for today

  • start cell lines
    • retrieve U2OS & SK-N-SH cell lines from freezer
    • thaw, resuspend in fetal bovine serum
    • transfer to growth medium in T25 flask
    • begin growth in chamber
  • Prepare new cell medium
    • SKNSH cell medium, contains:
      • Eagle's Modified Essential Medium (EMEM)
      • 10% fetal bovine serum (FBS)
      • 1x penicillin/streptomycin (from 100x stock)

Protocol See cell culture - mammalian, mammalian culture media, and thawing mammalian cells.

Notes U2OS and SKNSH cell lines started in T25 flasks. Placed in upper cell incubator, middle shelf.

To do next: tomorrow, split U2OS cell line into T75 flask.