Haynes Lab:Notebook/Protein-DNA fusions in living microbes/2013/11/09

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Reran gel of 9/21 experiment

Ran time course: pSB1C3 500ng 500ng 500ng 500ng 500ng 500ng 19.2uL 19.2uL 19.2uL 19.2uL 19.2uL 19.2uL topo eq. 0.5uL 0.5uL 0.5uL 0.5uL 0.5uL 1uL EcoRI

NEB 4 3uL 3uL 3uL 3uL 3uL 3uL ddH20 up to 30 total 30uL 30uL 30uL 30uL 30uL 30uL incubation:37C 4min 6 8 10 12 12+