Haynes Lab:Notebook/MPeterson Cloning Bootcamp/2015/01/29

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Session 2

Growing Liquid Cultures of Transformed Cells

Result for Plate 1: over 60 growth colonies; Result for Plate 2 (Negative Control): no growth

List of samples for liquid cultures: (1) Culture 1 = Colony 1 from Plate 1, DH5a-Turbo cells, KAH126/MV2 plasmid, Amp resistance (2) Culture 2 = Colony 2 from Plate 1, DH5a-Turbo cells, KAH126/MV2 plasmid, Amp resistance

(1) and (2) are each in 14-mL culture tubes an incubator to be left overnight, with an Amp agar plate with a streak of (1) and a streak of (2).