Haynes Lab:Notebook/Jan/2015/08/26

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Making Stable Cell Lines from U2OS Cells Transfected with BD004/BD006

  • Passaged cells at a 1:10, 1:20, 1:40, dilution into 10 cm plates with fresh antibiotic-free growth medium 48 hours after transfection on 8/24/15.
  • Total number was 6 10-cm plates: 3 with BD004 and 3 with BD006.
  • Also split U2OS cells for maintenance.