Haynes Lab:Notebook/Jan/2015/04/20

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Passaging U2OS Cells to Prepare for Creating a Stable Cell Line

  • Cell Line: U2OS
  • BD004 Benchling link:
  • BD006 Benchling link:
  • Protocol: Passage cells at a 1:10, 1:20, 1:40 dilution into fresh antibiotic-free growth medium 48 hours after transfection. We are passaging at 1:10, 1:20, and 1:40 of each plasmid into 10 cm plates.