Haynes Lab:Notebook/Jan/2015/03/23

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Transformation of DPRE/HoxD11.12

  • Plasmid name: DPRE
  • BioBrick name: BBa_J176033
  • Benchling link: DPRE
  • Ampicillin resistance
  • Length of plasmid: 1740 bp + 3200 bp V0120 backbone = 4940 bp
  • Transformation done on 3/23/2015
  • Result for Plate 1: Medium growth over the plate. Lack of high growth may be related to plasmid's age (last cloned in 2011). Overall success.
  • Result for Plate 2: No growth anywhere. Success.