H2B control (DNA, 10x buffer, Quick soln. in 25 μL; no PCR)
LOV mut rxn
LOV control (DNA, 10x buffer, Quick soln. in 25 μL; no PCR)
Add 1 μL DpnI enzyme to each sample
Gently and thoroughly mix each reaction, spin down in a microcentrifuge for 1 minute, and immediately incubate at 37°C for 1 hour to digest the parental supercoiled dsDNA
Transform 10 μl of the Dpn I-treated DNA from each control and sample reaction into separate 50-μl aliquots of BL21 cells