Haynes Lab:Notebook/Heathers Lab Notebook/2013/06/25

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Day One: Transformation of E Coli with plasmid DNA

  • started on 06/25/13
  • Plasmid was selected. 9μL of sterile H2O was put into tube. 1μL of selected plasmid was added. Competent cells were thawed on ice for ten minutes. 50μL of cells were pipetted into solution and placed back on the ice. Prewarmed agar plates were labeled with Amp resistance, the date 06/25/13, initials HB, the strain and DNA name. The total volume of cells (around 60μL were pipetted into agar plates. Sterile glass beads were used to spread the cells. The plates were than placed in the incubator at 37 degrees C.