Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2016/02/03

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Overview

Submitting samples for sequencing.

Sample ID Conc. (ng/µL) Amt for 150 ng (µL) Primer Primer amt (2 µL) H2O (µL) Sequencing # Sequencing ID
EED_v0120 1 123 1.2 P0001 2 2.8 1 1_EED_v0120 1_P0001
EED_v0120 1 123 1.2 P0002 2 2.8 2 2_EED_v0120 1_P0002
EED_v0120 3 99 1.5 P0001 2 2.5 3 3_EED_v0120 3_P0001
EED_v0120 3 99 1.5 P0002 2 2.5 4 4_EED_v0120 3_P0002
EM7:ZeoR_v0120 1 120 1.3 P0001 2 2.8 5 5_EM7:ZeoR_v0120 1_P0001
EM7:ZeoR_v0120 2 61 2.5 P0001 2 1.5 6 6_EM7:ZeoR_v0120 2_P0001
EM7:ZeoR_v0120 3 134 1.1 P0001 2 2.9 7 7_EM7:ZeoR_v0120 3_P0001
MV8 2 362 0.4 CMV fwd 2 3.6 8 8_MV8 2_CMV fwd
MV8 3 393 0.4 CMV fwd 2 3.6 9 9_MV8 3_CMV fwd
MV8 4 323 0.5 CMV fwd 2 3.5 10 10_MV8 4_CMV fwd