Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2016/01/28
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Using the QuikChange Site-Directed Mutagenesis Kit to fix the missense mutation in the ZeoR gene.
Prepare mixes (all units in µL):
Cycle using the following parameters:
Tomorrow will treat with DpnI, transform into XL1-Blue competent cells.