Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2016/01/11

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Overview

Starting liquid cultures of HA2_v0120. Inoculated 5 mL LB-Amp in culture tubes with cells from streaks 4 and 6. Once they reach exponential phase, will perform plasmid prep and then prepare for cloning next step and sequencing.

Plasmid Prep

Followed the instructions in the Sigma kit.

DNA Quantification

Measured concentrations are 162 ng/µL for culture 4, and 172 ng/µL for culture 6.