Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2016/01/11
|Today's project is...||Main project page|
Previous entry Next entry
Starting liquid cultures of HA2_v0120. Inoculated 5 mL LB-Amp in culture tubes with cells from streaks 4 and 6. Once they reach exponential phase, will perform plasmid prep and then prepare for cloning next step and sequencing.
Followed the instructions in the Sigma kit.
Measured concentrations are 162 ng/µL for culture 4, and 172 ng/µL for culture 6.