Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/12/15
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Phusion PCR of HPK:CFP part.
PCR protocol: basic Phusion, 60°C annealing temp, 90 second extension time, 35 cycles.
My PCR product is on the far right. Lots of non-specific binding and amplifcation. Main bands are at ~1500bp and ~2000bp. Expected product size is 2000bp.