Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/12/14
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Repeating PCR of pSB1A3, this time with the correct template.
Template:I13522_pSB1A3 Primers: P140, P141
PCR protocol: basic Phusion, 60°C annealing temp, 90 second extension time, 35 cycles.
See rightmost lane. Band visible in the correct size range. Looks like the PCR worked.
Performed PCR cleanup on pSB1A3_BsmBI, measured concentration using Take3 plate reader. Final concentration: 31.4 ng/µL