Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/11/24

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Overview

Waiting for new primers to arrive. In the meantime, attempted ligation of LCR product into pJET vector followed by transformation into DH5α-Turbo competent E. coli.

Method

Ligation

Used 3.5 µL of LCR product and 50 ng of pJET vector. Total ligation volume 10 µL.

Transformation

Two plates set up: sample containing cells transformed with ligation product, and negative control containing pJET only. Expect to see no colonies on pJET control due to kill gene.