Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/11/06

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Overview

Tried to make more of the HPK-CFP fragment by performing PCR on the gel extraction of previously amplified HPK-CFP. Results came out... interesting. Suspect that maybe the HPK-CFP fragment is the weak link in the LCR earlier this week.

2015-11-06 PCR of HPK-CFP gel extraction.jpg

Fragment should be approx. 2000bp in size... nothing there, just a smear.