Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/11/02

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Overview

PCR results: DNA quantification and gels.

DNA Quantification

Sample ID 260/280 ng/µL
HA1 (PCR cleanup) 1.839 149.34
HA2 (PCR cleanup) 1.868 127.448
EM7-ZeoR (PCR cleanup) 1.875 234.784
HPK-CFP (PCR cleanup) 1.877 236.064
HPK-CFP (gel extraction) 1.887 24.101

Gel Image

From left to right: 1kb+ ladder, HPK-CFP, HA1, HA2, EM7-ZeoR

2015-11-02 gel extraction of HPK-CFP.jpg

Cut out the large band from HPK-CFP and performed gel extraction, see above.