Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/10/29

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Overview

Phusion PCR results:

2015-10-29 HA1 ZeoR CFP HA2 phusion PCR analyzed.png

PCR on homology arm 1 and homology arm 2 worked fine. EM7-ZeoR and HPK-CFP fragments did not amplify. Going to repeat with gradient PCR.