Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/05/20

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Overview

Ordered new primers for PCR amplification of the DBN006 fragment. They're now matched to have similar melting temperatures and have a larger anchor on either side of the BsaI binding site. Primer names are DBN006 f1 and DBN006 r1.