Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/05/04
|Prep for cell cultures||Main project page|
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The Type IIS digestion/ligation followed by PCR didn't work out. I'm going to go ahead and grow up cultures from the BioBricks cloning of KAH57 & KAH104 and try a plasmid miniprep followed by digestion tomorrow.
For a total of 6 cultures.